The proportion of RB1 gene site pathogenic variant is higher in those with family genetic high-risk factors, binocular/trilateral RB, >12-month-onset RB, and in the high-risk group than those with no family history, unilateral eye RB, ≤12-month-old and low-risk group (P = 0.021, 0.001, 0.034, 0.049, respectively) Table 4. Here, RB1 is linked to retinoblastoma.