TNMplot [20] was further used for pan-cancer analysis of FLAD1, including 56,938 samples showing that FLAD1 is highly expressed in acute myeloid leukemi, bladder cancer, breast cancer, colon cancer, esophageal cancer, liver cancer, lung adenocarcinoma, lung squamous cell cancer, ovarian cancer, pancreatic cancer, rectum cancer, kidney renal papillary cell carcinoma, skin cancer, stomach cancer, testicular cancer, uterine carcinosarcoma, and uterine corpus endometrial carcinoma (Figure 3, p < 0.001). This evidence concerns the gene FLAD1 and esophageal cancer.