As the PI3K-AKT signaling pathway plays an important role in the regulation of tumor progression 26, 27, we then analyzed the relationship between CCDC65 and PI3K-AKT signaling pathway and found that CCDC65 significantly suppressed the levels of p-AKT1 (Ser473), but not total AKT1 protein (Figure 4B). Here, AKT1 is linked to neoplasm.