Clinically, the isolation and identification of PD-L1-positive CTCs have been introduced as a challenging issue in the case of NSCLC because neutrophils and other immature myeloid subsets have a low to absent expression of CD45 and also express PD-L1, which make it challenging to identify the CTCs and leads to potentially false-positive results 132. The gene discussed is CD274; the disease is non-small cell lung carcinoma.