GLS and posterior cortical atrophy: To analyze the effect of chemical inhibition of Gln metabolism on PCa radioresistance, four established PCa cell lines, DU145, 22Rv1, PC3 and LNCaP were treated for 48 h with LD50 doses of MYC inhibitor 10058-F4 or GLS inhibitor CB-839 and analyzed by radiobiological clonogenic assay (Figure 3C andFigure S4A-B).