To assess whether KDM4B is crucial for PCa cell proliferation, a lentiviral-based approach with the control (LKO) and two independent lines (sh4B#1 and sh4B#2) was utilized for KDM4B depletion in AR-positive LNCaP, C4-2B, and CWR22Rv1 cells (Figure 1B), resulting in significant reductions in cell proliferation [LNCaP: LKO vs. sh4B#1, p < 0.0001; LKO vs. sh4B#2, p < 0.0001; C4-2B: LKO vs. sh4B#1, p = 0.0043; LKO vs. sh4B#2, p = 0.0013; CWR22Rv1: LKO vs. sh4B#1, p = 0.0013; LKO vs. sh4B#2, p < 0.0001] (Figure 1C-E and S1A-C). Here, AR is linked to posterior cortical atrophy.