The IHC staining of HIF-1α, HDAC3, BCL3, and CCND1 and H&E staining was performed in the serial section of the HCC sample and we found the positive staining of HIF-1α, HDAC3, BCL3, and CCND1 in the same region (Supplementary Figure 6). This evidence concerns the gene HIF1A and hepatocellular carcinoma.