As shown in the experiments with ADNP knockdown in T24 and BIU87 BC cells, expression of TGF-β, TGF-βR1, Smad2, Smad3, p-Smad2 and p-Smad3 was significantly decreased, while overexpression of ADNP in 5637 and TCCSUP BC cells produced the opposite effects (Fig. 5). The gene discussed is SMAD2; the disease is breast cancer.