The results showed that the combined treatment significantly downregulated p-AKT and p-ERK in both cell lines but had no significant effect on the levels of AKT and ERK (Figures 3(b) and 3(c)), suggesting that HIF-1α RNAi and ASP regulate the expression of HIF-1α in HCC cells by inhibiting the MAPK and PI3K signaling pathways, thereby inhibiting the migration, invasion, and angiogenesis of these cells. The gene discussed is AKT1; the disease is hepatocellular carcinoma.