To assess the inhibitory effect of ASP combined with HIF-1α RNAi on migration and invasion, we treated HCC cells (SK-Hep1 and Hep-3B) with HIF-1α RNAi for 24 h (migration) or 48 h (invasion) together with ASP at 10 mg/mL. The gene discussed is HIF1A; the disease is hepatocellular carcinoma.