HIF1A and hepatocellular carcinoma: To verify whether the mechanism of ASP combined with HIF-1α RNAi inhibiting the proliferation, migration, invasion, and angiogenesis of HCC cells was correlated with HIF-1α and VEGF levels, the expression of HIF-1α and VEGF was assessed using qPCR (Figure 3(a)) and western blotting (Figures 3(b) and 3(c)).