To further clarify the mechanism by which HIF-1α RNAi + ASP inhibits the growth of subcutaneously transplanted HCC tumors in nude mice, IHC staining was performed in tumors from all groups (HIF-1α RNAi + ASP, HIF-1α RNAi, ASP, and control), and the expression of HIF-1α, VEGF, and CD34 in tumors was assessed. Here, HIF1A is linked to hepatocellular carcinoma.