In order to further explore the mechanism of HXHJ inducing apoptosis of HCC cells, our study combined in vivo and in vitro experiments; used immunofluorescence, western blot, and other methods; and found that HXHJ may regulate the expression of tumor cell signaling pathway p53/Bcl-2/Cyt-C/caspase-3, which induced mitochondrial cell apoptosis, thereby exerting a good anti-HCC effect. The gene discussed is TP53; the disease is neoplasm.