Combined with the previous research in CRC cells, the dissipation of ΔΨm, the activation of Bax, and downregulation of Drp1 suggest that the cell death induced by atractylenolide I could be regarded as intrinsic apoptosis, and atractylenolide I might inhibit the Drp1-mediated mitochondrial fission. The gene discussed is BAX; the disease is colorectal carcinoma.