The expression of NLRP3, caspase-1, and ASC, and the release of IL-1β were all suppressed by atractylenolide I. Furthermore, transfection with the Drp1 lentivirus vector significantly upregulated β-catenin and PCNA expression in the atractylenolide I–treated AOM/DSS mice model. The gene discussed is DNM1L; the disease is infectious otitis media.