It was found that in the pathophysiological process of ischemic stroke, PINK1/Parkin signaling pathway exerted a protective effect by mediating mitochondrial autophagy; it was also reported that the expression of LC3B and Beclin-1 was gradually up-regulated and reached the peak 24 h after cerebral ischemia-reperfusion, and the mitochondrial translocation of Parkin and the expression of PINK1 in mitochondrial outer membrane increased significantly at 24 h of reperfusion. The gene discussed is PRKN; the disease is ischemic stroke.