In the model of long-term ischemia or permanent middle cerebral artery occlusion (MCAO), the detection of mitochondrial autophagy-related proteins, including Beclin-1, LC3, p62, TOM20, and HSP60 also shows that mitochondrial autophagy is inhibited and that the volume of cerebral infarction increases significantly. Here, BECN1 is linked to brain infarction.