LASSO Cox regression analysis was carried out and identified 14 optimized autophagy-related genes, including ATG4A, BAK1, BNIP3, CALCOCO2, CCL2, DAPK1, EGFR, FAS, GRID2, ITGA3, MYC, RAB33B, USP10, and WIPI1, for the prognosis of osteosarcoma. This evidence concerns the gene EGFR and osteosarcoma.