LASSO Cox regression analysis was carried out and identified 14 optimized autophagy-related genes, including ATG4A, BAK1, BNIP3, CALCOCO2, CCL2, DAPK1, EGFR, FAS, GRID2, ITGA3, MYC, RAB33B, USP10, and WIPI1, for the prognosis of osteosarcoma. The gene discussed is ATG4A; the disease is osteosarcoma.