To better understand the role of TOLLIP in AKI after PQ intoxication, the expression of TOLLIP in rats and HK-2 cells was knocked down or overexpressed and the effect of TOLLIP knockdown or overexpression on the renal or cellular injury and NLR family pyrin domain-containing 3 (NLRP3) inflammasome in vivo and in vitro was subsequently investigated. This evidence concerns the gene TOLLIP and acute kidney injury.