To further understand whether LCN2 only potentiates the effects of inflammatory stimuli on FGF23 production in CKD or inflammation or whether LCN2 directly stimulates FGF23 production, we administered LCN2 to 6-week old WT mice either for 4 weeks using osmotic minipumps or during 4 days of repeated LCN2 injections. The gene discussed is LCN2; the disease is chronic kidney disease.