Re-expression of wild-type FBXO16 (FBXO16 WT), but not the F-box domain deleted mutant (FBXO16 ΔF) (Fig. 2G), can impair the proliferation, clonogenic survival, and cell invasion ability caused by FBXO16 deletion (Fig. 2H–J), indicating that the tumor inhibition effect of FBXO16 depends on its E3 ligase activity. Here, FBXO16 is linked to neoplasm.