Using four independent shRNA sequences targeting different regions of FBXO16 mRNA and three ovarian cancer cell lines with relatively high FBXO16 mRNA levels (A2780, OVCAR8, and SKOV3), we were able to achieve significant down-regulation levels of FBXO16 mRNA (Fig. 2B, Supplementary Fig. 1A). This evidence concerns the gene FBXO16 and ovarian cancer.