When WT, TLR 2(−/−), 4(−/−), and MyD88(−/−) BALB/c mice were IT challenged with OVA and/or AASD1, exacerbation of lung eosinophilia, increased Th2 cytokine and eosinophil-relevant chemokine production and induction of serum IgE and IgG were observed (He et al. 2016b). This evidence concerns the gene MYD88 and Increased total eosinophil count.