In order to track the melanoma cells using EGFP instead of melanin, we bred the sox10:EGFP fluorescent zebrafish line into our nf1/pten-mutant line to aid in visualization of the transplanted melanoma cells, as they expressed high levels of the neural crest progenitor marker sox10 (Supplementary Fig. S11) [24]. This evidence concerns the gene PTEN and melanoma.