Besides, simultaneous downregulation of BIRC5 and DEPDC1B could exacerbate the inhibitory effects of chordoma cell progression, which was characterized by inhibiting proliferation (P < 0.001, fold change = −3.3) (Fig. 5A), reducing clone formation (P < 0.001, fold change = −7.0) (Fig. 5B), stimulating apoptosis (P < 0.001, fold change = 14.1) (Fig. 5C) and repressing migration (P < 0.001, >70%) (Fig. 4D, E). The gene discussed is DEPDC1B; the disease is chordoma.