In a murine melanoma model, IL-35-producing Tregs accumulated in the TME and disrupted antigen-specific effector T cell activation and their effector function via falling them into the exhaustion.45 The expression pattern and immunosuppressive roles of IL-10 and IL-35 in tumor-infiltrating Tregs were different in tumor-bearing mice and patients with NSCLC.46 Tumor growth was slower in mice with IL-35-/-IL-10-/- Tregs compared with those with either IL-35-/- or IL-10-/- Tregs. This evidence concerns the gene IL10 and neoplasm.