Strong signals of cleaved PARP, cleaved Caspase-3 and cleaved Caspase-7 were detected in the recurrent chordoma cell lines U-CH1 and U-CH11R subsequent to HXR9 treatment confirming the previous results observed in the NucView Caspase-3/7 assay, whereas the signals were weak in the primary cell lines U-CH19 and U-CH11. The gene discussed is CASP7; the disease is chordoma.