HBV infection of liver organoids was dependent on NTCP as (pre)treatment with the competitive entry inhibitor myrcludex-B decreased infection of organoids as shown by quantitation of HBV intracellular RNA (Figure 3C) as well as HBV DNA (Figure 3D) and HBeAg (Figure 3E) in the supernatant of infected organoids. This evidence concerns the gene SLC10A1 and infection.