SPAM1 and neoplasm: To test whether enforced expression of the human hyaluronidase PH20 in T cells is able to confer an enhanced capacity to degrade an HA-containing tumor extracellular matrix, we constructed four types of lentiviral expression cassettes: two membrane-located forms with or without IgG2 Fc fragments and two secreted forms with or without IgG2 Fc fragments.