Flow cytometry assessing the response to OVA/alum in the spleen 7 days post-immunisation and x31-OVA in the mediastinal lymph node (mLN) 8 days post-infection, demonstrated that ~60% and ~45% of endogenous CD44hiCD8+ T cells expressed CXCR5, respectively, compared to naïve CD44loCD8+ T cells which displayed minimal positivity for CXCR5 (Figures 1A, B). The gene discussed is CXCR5; the disease is infection.