NUS1 and gestational diabetes: According to our findings, the C allele of SNP rs80196932 was associated with a lower risk of GDM and with higher expression of NUS1. Previous studies have indicated that NUS1 is essential for protein glycosylation and intracellular cholesterol trafficking (26), and another animal model-based study showed that mouse embryonic fibroblasts with conditional knockdown of Nus1 accumulated free cholesterol (17).