Furthermore, to elucidate mechanism of cantharidin in inhibiting osteosarcoma carcinogenesis and progression, we investigated expressions of DKK3, p-GSK-3β and active β-catenin in the total proteins, as well as the total β-catenin and LEF1 in the nuclear proteins of the cantharidin-treated osteosarcoma cells with or without CHIR99021, miR-214-3p over-expression or miR-214-3p silence. This evidence concerns the gene LEF1 and osteosarcoma.