LEF1 and osteosarcoma: However, biological functions and molecular mechanisms of cantharidin in osteosarcoma, including the accurate cell cycle arresting phase (G2 or M); the effect on colony formation, migration and invasion abilities, and EMT characteristics; the involvement of miR-214-3p/DKK3/GSK-3β/β-catenin/LEF1 signaling pathway; the in vivo (using the orthotopic xenograft mouse model) anti-osteosarcoma efficacy need to be further investigated.