Western blotting assay revealed that overexpression of Cx43 sufficiently inhibited c-Src phosphorylation (Fig. 10B), indicating that the interaction of Cx43 with c-Src reduces c-Src activity, which is consistent with the result that Cx43 and c-Src interaction suppressed c-Src activity in glioma stem cells 65. The gene discussed is SRC; the disease is glioma.