To investigate if PRL3-zumab prevents tumor repopulation after surgery in these mice by eradicating rapidly evolving PGCC-like cells, we analyzed the difference in cell morphology and DNA content in frozen tumor sections derived from both the original (“primary”) and post-surgical relapsed tumors (“relapse”) of the animal models we used in Fig. 4, B16F0 and SNU-484 tumors. The gene discussed is PTP4A3; the disease is neoplasm.