Significantly, in A549 cells and other primary NSCLC cells (pNSCLC2 and pNSCLC3, see Fig. 2), sh-POLRMT-S1-induced silencing of POLRMT led to mitochondrial depolarization (JC-1 green monomer intensity increase, Fig. 3F) and apoptosis activation (TUNEL-positive nuclei ratio increase, Fig. 3G). This evidence concerns the gene POLRMT and non-small cell lung carcinoma.