RNAseq measurements from parasites subjected to this induction trigger showed an upregulation of 295 gene transcripts, including those encoding proteins associated with bradyzoites, such as the cyst wall antigens CST1 and MAG1, the stage-specific enzymes ENO1 and LDH2, and AP2 transcription factors associated with early bradyzoite development. This evidence concerns the gene CST1 and cyst.