In this regard, the Gini index calculation for TCRα and TCRβ for the whole CD3+ T cell population was unaltered after ld-IL-2 treatment or in samples from the Treg-T1D trials, suggesting that effects of ld-IL-2 treatment were manifested in the PRF1+GZMB+CD8+ T cell subset selectively (Figure 7A). Here, PRF1 is linked to type 1 diabetes mellitus.