The results of glycolysis cell-based assay also verified that the lactic acid content increased after the expression of PAICS was up-regulated, suggesting that the up-regulated expression of PAICS may promote the glycolysis process, provide intermediate products for the proliferation of NSCLC cells, and play a role in promoting cancer, this is consistent with the results of PAICS overexpression in lung adenocarcinoma [29]. The gene discussed is PAICS; the disease is cancer.