For Annexin V/PI flow cytometry, under the condition of perifosine treatment, the apoptotic rate of NB cells with ATXN3 siRNAs transfected was significantly higher than control group (with perifosine treatment, in AS cells, ATXN3 siRNA #1 and #2 vs. control siRNA: 24.7% and 29.8% vs. 16.8%, P < 0.01, Figure 1F; in BE2 cells, ATXN3 siRNA vs. control siRNA: 27.7% vs. 15.9%, P < 0.01, Figure 1G). This evidence concerns the gene ANXA5 and neuroblastoma.