To investigate the potential mechanism by which the downregulation of ATXN3-enhanced perifosine-induced cell death in NB cells, we knocked down ATXN3 by transfecting ATXN3 siRNA (control siRNA, #1, #2, and #3) into AS cells, and screened the expression changes of BCL-2 family members, then we performed protein quantitative analysis. This evidence concerns the gene BCL2 and neuroblastoma.