For Annexin V/PI flow cytometry, under the condition of etoposide treatment, the apoptotic rate of NB cells with ATXN3 siRNAs transfected was significantly lower than the control group (with etoposide treatment, in AS cells, ATXN3 siRNA #1 and #2 vs. control siRNA: 24.1% and 27.7% vs. 38.4%, P<0.001, Figure 4E, right; in BE2 cells, ATXN3 siRNA vs. control siRNA: 19.1% vs. 28.8%, P<0.01, Figure 4F, right). This evidence concerns the gene ATXN3 and neuroblastoma.