The characteristic IHC photos of prognostic genes in tumor and normal tissues are presented in Figure 8B and the results indicated that six of the prognostic genes showed significant downregulation in COAD compared with normal tissue, including MIER3, CHGA, SLC41A2, NAT1, NAT2, and ZG16. However, the HPA dataset did not provide the immunochemical profiles of HRCT1, LINCO1133, and PPARGC1A. Moreover, we employed Pearson correlation analysis to explore the correlation between the mRNA expressions of the 13 prognostic genes in the TCGA dataset. Here, ZG16 is linked to neoplasm.