ZG16 and neoplasm: All 13 genes were determined to be downregulated at the genetic level in COAD tissues relative to normal samples, among which six genes were consistent with the IHC results in the HPA dataset and tended to be reduced at the protein level in tumor specimens, including MIER3, CHGA, SLC41A2, NAT1, NAT2, and ZG16, providing the vital function of favorable prognostic genes in COAD.