Next, we constructed a novel gene-based signature consisting of 13 genes (FXYD3, MIER3, LINC01133, CHGA, TSPAN1, HRCT1, FRMD3, NR3C2, SLC41A2, NAT1, NAT2, ZG16, and PPARGC1A) for predicting the OS of COAD patients. This evidence concerns the gene ZG16 and colon adenocarcinoma.