Next, we constructed a novel gene-based signature consisting of 13 genes (FXYD3, MIER3, LINC01133, CHGA, TSPAN1, HRCT1, FRMD3, NR3C2, SLC41A2, NAT1, NAT2, ZG16, and PPARGC1A) for predicting the OS of COAD patients. Here, TSPAN1 is linked to colon adenocarcinoma.