TLR4 and clostridium difficile infection: In this study, TLR4 polymorphism was not associated with the development of CDI; however, in our published study, the TLR4 rs1927914 polymorphism (GG genotype) was associated with C. difficile colonization, suggesting that TLR4 polymorphism might lead to adhesion or adaption but does not induce inflammation against C. difficile in the gut (23).