Tlr2-/- and Tlr4-/- mice both showed more severe CDI symptoms than control mice in terms of body weight change (with a 1.10 g decrease in Tlr2-/- mice and a 1.38 g decrease in Tlr4-/- mice, compared to a 0.74 g decrease in wild-type mice, Figure 2A) and fecal content (Figure 2C) on the 5th day after challenge with C. difficile. Here, TLR4 is linked to clostridium difficile infection.