Upregulation of PIK3R3 and the cancer stem cell marker CD133 in spheroid cells was verified using quantitative real-time PCR (qRT-PCR) in HT1080 and SK-LMS-1 cells as well as DDLS8817 dedifferentiated liposarcoma cells, which showed 3.9–6.2 and 2.1–3.8-fold higher expression, respectively, in spheroid vs. monolayer cells (Fig. 1B). The gene discussed is PIK3R3; the disease is cancer.