Likewise, in this study the treatment of lung cancer H460 cells with cisplatin at the maximal response concentration (50 μM) clearly stimulated autophagy, as indicated by the conversion of LC3-I to LC3-II, formation of autophagosomes, and lysosomal degradation of the autophagic substrate, p62 (Fig. 2). This evidence concerns the gene SQSTM1 and lung carcinoma.