Furthermore, treatment with the PI4K2ɑ-specific inhibitor PI-273 (Li et al., 2017) suppressed accumulation of LC3 and ubiquitin, which serve as markers for autophagosomal maturation, on Salmonella in SACM1L KO cells at 2 h post-infection (Figures 5J and 5K). This evidence concerns the gene SACM1L and infection.