We monitored the dynamic recruitment of GFP-tagged Gal3 to intracellular dsRed-expressing Salmonella (Maejima et al., 2013; Rioux et al., 2007) but did not detect differences in co-localization in WT and SACM1L KO cells, suggesting that the loss of SAC1 does not affect SCV integrity at 1 h post-infection (Figures S2H and S2I). Here, SACM1L is linked to infection.