To evaluate Salmonella existing within these compartments, we monitored WT and SACM1L KO cells co-expressing GFP-LC3 (marking autophagosomes) and LAMP1-mCherry (marking SCVs) by live cell imaging for 6 h post-infection (Lane et al., 2019; Valdivia and Falkow, 1996). This evidence concerns the gene LAMP1 and infection.