SACM1L and infection: The percentage of Salmonella-containing autophagosomes with detectable PI(4)P levels (i.e., GFP-LC3+BFP-2xP4M+Salmonella) in SACM1L KO cells was initially similar to WT cells, which remained below 5% but reached a maximum of 12% by 150 min post-infection (Figures 5F and 5G), demonstrating that PI(4)P accumulates on Salmonella-containing autophagosomes upon SAC1 loss.