Previously, we evaluated the effects of DNA demethylating agent 5-aza-CdR on DNMT1 gene expression and apoptosis induction and also cell viability in the HCC WCH-17 cell line [Kavoosi et al., 2019; Sanaei et al., 2020; Sanaei et al., 2019). Here, DNMT1 is linked to hepatocellular carcinoma.