Finally, the levels of mRNA transcripts of these DEGs among the purified parental CD44+/CD24- CSCs (red color), the newly converted CD44+/CD24- CSCs (blue color) and the unconverted CD44-/CD24- tumor cells (green color) from MDA-MB-231 cells were tested by RT-qPCR. The gene discussed is CD44; the disease is neoplasm.