(E–H’) Longitudinal X-ray analysis of a representative Col2a1-Cre; Adgrg6f/f mutant mouse at P10 (E, E’), P20 (F, F’), P40 (G, G’), and P120 (H, H’), showing adolescent-onset (F) and progressive (F–H) thoracic scoliosis, with the apex of scoliosis indicated (red arrows; F–H’). The gene discussed is COL2A1; the disease is Thoracic scoliosis.