For instance, rarer alternative EGFR splicing events were detected in the unexplained set, such as EGFRvIII with an alternate exon 1 joined to exon 8 (chr7:55161631–55172981), detected in two patients with GBM and one patient with low-grade glioma, the same alternate exon 1 joined with two alternate exon 16s (chr7:55161631–55168521 and chr7:55161631–55170305, detected in one and two GBM patients, respectively) and the same alternate exon 1 joined with exon 20 (chr7:55161631–55191717) in two GBM patients. Here, EGFR is linked to central nervous system cancer.