To study this issue further, we evaluated the effects of pan-ROCK-i, Rip and ROCK2-i, KD025 on physical properties, size and stiffness, and mRNA expressions of ECM and their regulatory factors including TIMP and MMP of a recently established in vivo 3D HTM sphenoid culture model replicating glaucoma TM20 in addition to the conventional 2D culture of HTM cells. Here, TIMP1 is linked to glaucoma.