To further observe the protective effect of STING agonist on bone cancer pain, we used a more physiological model of bone metastasis by administering LLC cells (200,000 in 100 μl) via intra-caudal artery injection (Supplementary Fig. 8a), which was recently reported to deliver cancer cells to the hind limbs and caudal vertebrae with high efficacy and cause bone destruction46. This evidence concerns the gene STING1 and bone cancer.