CDK12 and posterior cortical atrophy: To better imitate the real dose of AR antagonists administered to patients [43, 44], we treated PCa cells with 25 μM AR antagonists, and found that a low dose of 0.2 μM THZ531 was enough to induce striking synergy in colony formation assays (Fig. 5B), supporting the essential requirement of CDK12 for PCa cells under AR antagonism.