In this study, the expression of genes related to ICIs was increased in the high-risk score patients with colon cancer, such as PDCD1, HAVCR2, CTLA4, CD8A, CXCL9, LAG3, TBX2, and PRF1. In addition, in the high autophagy-related risk patients, most of the genes negatively mediating the Cancer-Immunity Cycle were highly expressed, which also further promoted immunosuppression. Here, PRF1 is linked to colonic neoplasm.