ESR1 and neoplasm: We observed that more than 3 ER-α+ tumor samples exhibited mean ER-α66 expression level (μ), measured in fluorescence area-under-curve, AUC (μER-α66_32818–5 = 0.97 × 106, μER-α66_4318–1 = 1.09 × 106, μER-α66_1216 = 1.09 × 106, μER-α66_0225 = 1.46 × 106; nER-α66_32818–5 = 5 cells, nER-α66_4318–1 = 9 cells, nER-α66_1216 = 59 cells, nER-α66_0225 = 55 cells; Fig 2A).