TNF and viral infectious disease: After the mice were sacrificed and then bled, the serum samples were incubated with precoated beads, and flow cytometry results showed that the levels of key inflammatory factors, including Mcp-1, IL-12p70, IFN-γ, and TNF-α were consistently decreased in the Rtn3-overexpressing mice upon viral infection, especially IFN-γ and TNF-α, both of which were markedly downregulated (Figure 6E).