(B) CoIP and immunoblot analysis of HEK293T-shCtrl and HEK293T-shTRIM25 cells transfected with HA-Ub-K63 and increasing amounts of Flag-RTN3 (+, ++) or Flag-Ev together with GFP-RIG-I or GFP-Ev in the indicated groups for 24 hr and followed by infection with VSV (MOI = 1) for 8 hr. The gene discussed is RTN3; the disease is infection.