Fluorescence microscopy and flow cytometry results showed that RTN3 overexpression rendered the cells highly susceptible to viral infection and augmented virus amplification compared to the empty vector-transfected cells, which displayed a less sensitive phenotype (Figure 2C and D), and the MTS assay performed with control cells and RTN3-overexpressing HEK293T cells for over 36 hr showed that the cell viability was not affected by RTN3 overexpression (Figure 2—figure supplement 1E). This evidence concerns the gene RTN3 and viral infectious disease.