HMOX1 and infection: To further eliminate the interference of cytoplasmic HO-1 and to verify the sole role of nuclear HO-1 in regulating endothelial senescence, HO-1 was knocked down by CRISPR/Cas9, followed by the infection of recombinant adenovirus encoding truncated HO-1 without TMS but with three nuclear localization sequences (NLS: KRPAATKKAGQAKKKK ×3).