In accordance with the longstanding view that the kinetics of prion disease onset are inversely proportional to levels of PrP expression in the CNS [46,47], our previous studies showed that intracerebral (ic) inoculation with NA CWD prions produced disease more rapidly in Tg mice compared to their Gt mouse counterparts expressing physiologically accurate but lower levels of PrP [39]. This evidence concerns the gene PRNP and prion disease.